The Action of Phenylthiocarbamide on Tyrosinase
نویسنده
چکیده
Richter and Clisby (1) have investigated the t,oxic effect of phenylthiocarbamide in rats. The acute toxic effect is characterized by an effusion of fluid into the thoracic cavity, but in chronic poisoning there is a lowered body temperature, which suggests a depression of metabolism, and compensatory changes in the thyroid. It was therefore of interest to test the effect of the drug in vitro on isolated enzyme systems. Further, Richter and Clisby (2) observed graying of the hair in black rats after feeding phenylthiocarbamide to them for 2 or 3 months. This suggested an interference with melanin formation, and for this reason we investigated the effect of the drug on tyrosinase. Phenylthiocarbamide is a very effective inhibitor for tyrosinase, even in concentrations as low as 1 X lo4 M, and the characteristics of this inhibition are described. The experiments with animal enzyme preparations have yielded negative results.
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